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imagestream x mark ii imaging flow cytometer  (Merck & Co)

 
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    Structured Review

    Merck & Co imagestream x mark ii imaging flow cytometer
    Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by <t>ImageStream</t> ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01
    Imagestream X Mark Ii Imaging Flow Cytometer, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagestream+x+mark+ii+imaging+flow+cytometer/cytometer+easycyte+flow+guava/pmc12911089-61-11-19
    Average 86 stars, based on 1 article reviews
    imagestream x mark ii imaging flow cytometer - by Bioz Stars, 2026-10
    86/100 stars

    Images

    1) Product Images from "Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion"

    Article Title: Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion

    Journal: Cancer Cell International

    doi: 10.1186/s12935-025-04118-w

    Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by ImageStream ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01
    Figure Legend Snippet: Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by ImageStream ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01

    Techniques Used: Isolation, Purification, Cell Culture, Incubation, Flow Cytometry, Labeling, Fluorescence, Imaging

    Related Articles

    other:

    Article Title: Microtubule-Targeted Self-Assembly Triggers Prometaphase-Metaphase Oscillations Suppressing Tumor Growth.
    Article Snippet: The viscosity of H2O/glycerol mixtures was first measured using a Modular Compact Rheometer (MCR 302, Anton-Paar) at 25°C .

    Imaging:

    Article Title: Kojic Acid Derivative as an Antimitotic Agent That Selectively Kills Tumour Cells.
    Article Snippet: .. The ImageStream®X Mark II Imaging Flow Cytometer (Merck Life Science Limited, Vale RoadArklowCo, Wicklow, Ireland) was used for the analysis. .. Moreover, live cell imaging was prepared according to the manufacturer’s instructions, and the image analysis was prepared with Zoe® fluorescence microscopy (Bio-Rad Laboratories, Via Benvenuto Cellini, 18/A, 20054 Segrate (MI), Italy).

    Article Title: Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion
    Article Snippet: For cellular imaging illustration, CFSE-labeled K562 cells, both with and without platelets, were stained with CD61/PerCP-Cy-5.5 (BD Biosciences). .. Subsequently, fluorescent images of each cell were directly acquired using the ImageStream ® X Mark II imaging flow cytometer (Merck) and Confocal microscopy (Leica). ..

    Flow Cytometry:

    Article Title: Kojic Acid Derivative as an Antimitotic Agent That Selectively Kills Tumour Cells.
    Article Snippet: .. The ImageStream®X Mark II Imaging Flow Cytometer (Merck Life Science Limited, Vale RoadArklowCo, Wicklow, Ireland) was used for the analysis. .. Moreover, live cell imaging was prepared according to the manufacturer’s instructions, and the image analysis was prepared with Zoe® fluorescence microscopy (Bio-Rad Laboratories, Via Benvenuto Cellini, 18/A, 20054 Segrate (MI), Italy).

    Article Title: Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion
    Article Snippet: For cellular imaging illustration, CFSE-labeled K562 cells, both with and without platelets, were stained with CD61/PerCP-Cy-5.5 (BD Biosciences). .. Subsequently, fluorescent images of each cell were directly acquired using the ImageStream ® X Mark II imaging flow cytometer (Merck) and Confocal microscopy (Leica). ..

    Confocal Microscopy:

    Article Title: Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion
    Article Snippet: For cellular imaging illustration, CFSE-labeled K562 cells, both with and without platelets, were stained with CD61/PerCP-Cy-5.5 (BD Biosciences). .. Subsequently, fluorescent images of each cell were directly acquired using the ImageStream ® X Mark II imaging flow cytometer (Merck) and Confocal microscopy (Leica). ..



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    Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by <t>ImageStream</t> ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01
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    Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by <t>ImageStream</t> ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01
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    Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by ImageStream ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01

    Journal: Cancer Cell International

    Article Title: Interferon-α enhances NK cell function to counteract autologous platelet-mediated tumor immune evasion

    doi: 10.1186/s12935-025-04118-w

    Figure Lengend Snippet: Experimental strategy for evaluating autologous platelet effects on anti-tumor immunity of NK cells. ( A ) K562 cells were treated with platelets freshly isolated from healthy donors at a ratio of 1:500 for 3 h. Simultaneously, NK cells were purified from the same blood donors, then co-cultured with the K562 cells, which had been pre-treated with or without platelets, at a ratio of 4:1. After 16-hour incubation, we evaluated the anti-tumor responses of NK cells and the viability changes of K562 cells using flow cytometry. ( B ) Gating strategy for K562 cells. We gated on K562 cells and applied FSC-A versus FSC-H parameters to exclude doublets before analyzing CD61 signals. ( C ) CD61 + platelets on CFSE-labeled K562 cells. Representative plots (upper panel) show the percentage of CD61 + fluorescence on CFSE + K562 cells. Histograms (lower panel) display the MFI of CD61 on CFSE + K562 cells. ( D ) Summary of CD61 positivity of CFSE + K562 cells with or without platelet pre-treatment ( n = 10). ( E ) Fluorescent photographs of CD61 (PerCP-Cy5.5, red signal, excitation/emission: 488 nm/695 nm)- and CFSE (green signal, excitation/emission: 488 nm/518–521 nm) - labeled cells with or without platelets pre-treatment were taken by ImageStream ® X Mark II imaging flow cytometer. MFI, mean fluorescence intensity; **, p < 0.01

    Article Snippet: Subsequently, fluorescent images of each cell were directly acquired using the ImageStream ® X Mark II imaging flow cytometer (Merck) and Confocal microscopy (Leica).

    Techniques: Isolation, Purification, Cell Culture, Incubation, Flow Cytometry, Labeling, Fluorescence, Imaging

    Journal: iScience

    Article Title: Imaging flow cytometry reveals divergent mitochondrial phenotypes in mitochondrial disease patients

    doi: 10.1016/j.isci.2024.111496

    Figure Lengend Snippet:

    Article Snippet: ImageStream®X Mark II Imaging Flow Cytometer , Millipore , .

    Techniques: Derivative Assay, Control, Recombinant, Software, Imaging, Flow Cytometry, Spectrophotometry